Let's say we are sequencing total CD3+ cells in a tissue, and my population of interest is rare, i.e., 1%. How many cells should I sequence and have enough of my rare cell population? This is a power analysis. I will refer you to this helpful online tool by Satija's lab: https://satijalab.org/howmanycells/ For example, if tetramer+ cells are 1% of my CD3+ T cells and we want to sequence at least 20 cells, we need about 3,000 cells to have a ~95% chance of capturing 20 tetramer+ cells.

Here is the Rosetta viewer! We deposited recent T cell profiling by CITE-seq from baseline tissues. Please be patient with Rosetta, work in progress! Please allow 10-30s for the data to load before selecting samples. https://cbdm.connect.hms.harvard.edu/Rosetta_ImmGenT/