1. Make sure they are represented in enough numbers (pool mice, enrich, etc.).
2. In the spirit of profiling all T cells in ImmGen T, we would also suggest profiling all CD3+ cells as one of the samples. Will serve as reference of the range of states that cells can adopt, background repertoire analysis, etc.
3. Don't forget the spleen whole CD45+ control when you plan the experiment and fill the sample prep excel sheet.