Since it's at the single cell level, do all experiments need to be done in Boston or could some of them be profiled locally and then the raw data send to Immgen ?
Yes, this program will be trickier than conventional RNAseq, for which frozen lysates travel easily.
Principally, there will be two main immgenT sites. One in San Diego and one in Boston, who will use the same reagents and strict SOPs. From there, several scenarios are possible, depending on the actual types of samples: for "easy" cases, simply send the mouse protocol/mutants to one of the two sites, and the preparations will be performed by the core team; if more complex, investigators go to Boston or San Diego to prep and run tricky samples*. There is also the outside possibility of having additional sites runs the cell preparations and encapsulation (not preferred, in order to limit experimental drift)
Yes, this program will be trickier than conventional RNAseq, for which frozen lysates travel easily.
Principally, there will be two main immgenT sites. One in San Diego and one in Boston, who will use the same reagents and strict SOPs. From there, several scenarios are possible, depending on the actual types of samples: for "easy" cases, simply send the mouse protocol/mutants to one of the two sites, and the preparations will be performed by the core team; if more complex, investigators go to Boston or San Diego to prep and run tricky samples*. There is also the outside possibility of having additional sites runs the cell preparations and encapsulation (not preferred, in order to limit experimental drift)
* this will happen for "analysis jamborees"